کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3169139 | 1199458 | 2007 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Genetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic agents Genetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic agents](/preview/png/3169139.png)
ObjectiveFormocresol, paramonochlorophenol, or calcium hydroxide have been widely used in dental practice to eradicate bacteria and consequently to produce root canal disinfection. Taking into consideration strong evidence for a relationship between DNA damage and carcinogenesis, the purpose of the present study was to evaluate the genotoxic effects of antimicrobial endodontic compounds in human peripheral lymphocytes by single-cell gel (comet) assay. This technique detects DNA strand breaks in individual cells.Study designA total of 10 μL of the tested substance solution (formocreso1, paramonochlorofeno1, and calcium hydroxide at 100-μg/mL concentration) was added to human peripheral lymphocytes from 10 volunteers for 1 hour at 37°C. The negative control group was treated with vehicle control (PBS) for 1 hour at 37°C, as well. For the positive control group, lymphocytes were exposed to hydrogen peroxide at 100 μM during 5 minutes on ice.ResultsNo DNA breakage was detected after a treatment of peripheral lymphocytes by formocresol, paramonochlorophenol, or calcium hydroxide at 100 μg/mL.ConclusionsIn summary, our results indicate that exposure to formocresol, paramonochlorophenol, or calcium hydroxide may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single-cell gel (comet) assay.
Journal: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology - Volume 104, Issue 2, August 2007, Pages e58–e61