کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3169405 1199476 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of bacteria associated with spreading odontogenic infections by 16S rRNA gene sequencing
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Identification of bacteria associated with spreading odontogenic infections by 16S rRNA gene sequencing
چکیده انگلیسی

ObjectiveTo determine the bacterial species associated with spreading odontogenic infections (SOIs).Study designPus samples from 4 cases of SOI were analyzed by microbiological culture methods for the presence of bacteria, and by polymerase chain reaction (PCR) amplification, cloning, and sequencing of bacterial 16S rRNA genes.ResultsCulture methods identified species from the genera Prevotella, Streptococcus, and Fusobacterium, as well as anaerobic streptococci. Molecular detection methods identified a far more diverse microflora. The predominant genus detected was Prevotella, representing 102 (50.2%) of 203 clones analyzed. Prevotella oris was the most abundant species identified, representing 45 (22.2%) of 203 clones analyzed. Twelve clones (5.9%) represented uncultivable species, namely Prevotella PUS9.180, an uncultured Peptostreptococcus species, and an uncultured bacterium belonging to the Bacteroidetes phylum.ConclusionsPrevotella species may play an important role in SOIs, and further work to examine in more detail the pathogenicity determinants of these organisms and associated host responses is warranted.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology - Volume 103, Issue 5, May 2007, Pages 610–617
نویسندگان
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