کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3169768 1199496 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of an enamel matrix derivative on human osteoblasts and PDL cells grown in organoid cultures
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Effects of an enamel matrix derivative on human osteoblasts and PDL cells grown in organoid cultures
چکیده انگلیسی

ObjectiveThe objective of this study was to investigate cellular effects of enamel matrix derivative (EMD) in human derived, primary osteoblasts and periodontal ligament (PDL) cells grown in organoid cultures.Study designCell replication was assessed by BrdU-incorporation. [3H]-proline incorporation was measured to determine the synthesis of proline-containing proteins, such as collagen. In addition, calcium accumulation and alkaline-phosphatase-activity were quantified. Electron microscopy for morphological analysis was performed.ResultsOur results showed that EMD enhances BrdU-incorporation in PDL cells and osteoblasts. Also, in osteoblast organoid cultures [3H]-proline incorporation was 3-fold increased (P < .01). Extensive matrix deposition was noted in osteoblast cultures by electron microscopy. In osteoblasts, high levels of calcium accumulation and alkaline-phosphatase-activity were found. However, EMD did not promote mineralization.ConclusionOur results indicate that under organoid culture conditions EMD is able to promote the synthesis of proline-containing proteins such as collagen but not matrix mineralization of primary human osteoblastic cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology - Volume 102, Issue 4, October 2006, Pages 551–557
نویسندگان
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