کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3169825 1199497 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate
چکیده انگلیسی

ObjectiveTaking into consideration that DNA damage plays an important role in carcinogenesis, the purpose of this study was to evaluate whether regular and white mineral trioxide aggregate (MTA) are able to induce genetic damage in primary human cells.Study designHuman peripheral lymphocytes obtained from 10 healthy volunteers were exposed to 2 presentation forms of MTA at final concentrations ranging from 1 to 1000 μg/mL for 1 hour at 37°C. The negative control group was treated with vehicle control (phosphate buffer solution, PBS) for 1 hour at 37°C and the positive control group was treated with hydrogen peroxide (at 100 μM) for 5 minutes on ice. Results were analyzed by the Friedman nonparametric test.ResultsThe results pointed out that either regular or white MTA in all concentrations tested did not induce DNA breakage in human peripheral lymphocytes as depicted by the mean tail moment.ConclusionIn summary, our results indicate that exposure to MTA may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single cell gel (comet) assay.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology - Volume 102, Issue 5, November 2006, Pages e50–e54
نویسندگان
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