In vitro evaluation of macrophage viability after incubation in orange oil, eucalyptol, and chloroform

ObjectiveThe aim of this study was to evaluate the cytotoxicity of orange oil, eucalyptol, and chloroform in a cell culture assay by using peritoneal macrophages from Swiss mice.Study designControl (Dulbecco’s modified Eagle’s medium [DMEM] plus 1.25% ethyl alcohol) and experimental (orange oil, eucalyptol, and chloroform) groups were studied. Solvents used were tested at 0.025% and 0.050% concentrations in DMEM plus 1.25% ethyl alcohol.ResultsOrange oil, eucalyptol, and chloroform were all cytotoxic in comparison to the control group (P < .001). Orange oil showed the least cytotoxicity (P < .001). No significant differences were observed regarding cell viability when comparing the eucalyptol and chloroform groups (P < .05). There were significant differences in the cytotoxicity of eucalyptol and chloroform with an increase in concentration (P < .0001). Nevertheless, this difference was not significant in the orange oil group (P < .05).ConclusionOrange oil was less cytotoxic than eucalyptol and chloroform.