کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
31707 44831 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of galactose on direct and indirect pathway estimates of hepatic glycogen synthesis
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Effects of galactose on direct and indirect pathway estimates of hepatic glycogen synthesis
چکیده انگلیسی

Hepatic glycogen is formed by direct and indirect pathways whose activities reflect altered nutrition or disease. Direct/indirect pathway measurements often involve test meals where ∼10% of carbohydrate is galactose, but its effects on direct/indirect pathway estimates are unknown. Therefore, direct/indirect pathway contributions in 24-h fasted rats given 2 g/kg 100% glucose (GLU, n=6) or 90% glucose–10% galactose (GLU+GAL, n=6) were measured by [U-13C]glucose dilution and by position-5/position-2 glycogen enrichment (H5/H2) from 2H2O. For GLU+GAL, galactose glycogenesis was independently measured with [1-13C]galactose. Glycogenesis was equivalent in both groups but for GLU+GAL, 23±4% of glycogen was derived from galactose. [U-13C]glucose reported a 30±3% direct pathway contribution to glycogenesis for GLU but only 20±3% for GLU+GAL (p=0.012 vs. GLU). H5/H2 yielded identical direct pathway estimates (32±3% GLU, 29±6% GLU+GAL). Thus, galactose glycogenesis was undetected by H5/H2 while [U-13C]glucose reported a reduced direct/indirect pathway ratio. With [1-13C]galactose also present, correct glycogenic source contributions were obtained.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 12, Issue 6, November 2010, Pages 552–560
نویسندگان
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