کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
31909 44851 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The Acinetobacter sp. chnB promoter together with its cognate positive regulator ChnR is an attractive new candidate for metabolic engineering applications in bacteria
چکیده انگلیسی

Over the last 10 years there has been an extremely fast development in the global characterization of bacteria at the genome, transcriptome, proteome and metabolome levels. To further explore and apply these complex data sets there is now a need for new biological tools that can be used to test or verify hypotheses generated on the basis of all the new information. Here, we report the integration of an expression cassette based on the Acinetobacter sp. chnB promoter and its cognate positive regulator chnR gene into a replicon derived from the broad-host-range plasmid RK2. Cyclohexanone was found to be the most efficient inducer of this system in Escherichia coli, using firefly luciferase as a reporter. To explore the potential of the system in another species, we show that the system can be used in combination with another similar expression cassette (Pm/xylS) to control the monomer composition of the industrially widely used exopolysaccharide alginate, produced by Pseudomonas fluorescens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 10, Issue 2, March 2008, Pages 121–129
نویسندگان
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