کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
31994 44872 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recombinant protein production in an Escherichia coli reduced genome strain
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Recombinant protein production in an Escherichia coli reduced genome strain
چکیده انگلیسی

Recently, efforts have been made to improve the properties of Escherichia coli as a recombinant host by ‘genomic surgery’—deleting large segments of the E. coli K12 MG1655 genome without scars. These excised segments included K-islands, which contain a high proportion of transposons, insertion sequences, cryptic phage, damaged, and unknown-function genes. The resulting multiple-deletion strain, designated E. coli MDS40, has a 14% (about 700 genes) smaller genome than the parent strain, E. coli MG1655. The multiple-deletion and parent E. coli strains were cultured in fed-batch fermenters to high cell densities on minimal medium to simulate industrial conditions for evaluating growth and recombinant protein production characteristics. Recombinant protein production and by-product levels were quantified at different controlled growth rates. These results indicate that the multiple-deletion strain's growth behavior and recombinant protein productivity closely matched the parent stain. Thus, the multiple-deletion strain E. coli MDS40 provides a suitable foundation for further genomic reduction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 9, Issue 2, March 2007, Pages 133–141
نویسندگان
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