کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
31996 | 44872 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli Evolution of a Saccharomyces cerevisiae metabolic pathway in Escherichia coli](/preview/png/31996.png)
چکیده انگلیسی
The Saccharomyces cerevisiae glycerol pathway (GPD1 and GPP2) was evolved in vivo in Escherichia coli. The central metabolism of E. coli was engineered to link glucose consumption and glycerol production. The engineered strain was evolved in a chemostat culture and a high glycerol producer was rapidly obtained. The evolution of the strain was associated to a deletion between GPD1 and GPP2, resulting in the production of a fusion protein with both glycerol-3-P dehydrogenase and glycerol-3-P phosphatase activities. The higher efficiency of the fusion protein was due to partial glycerol-3-P channeling between the two active sites. The evolved strain produces glycerol from glucose at high yield, concentration and productivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Metabolic Engineering - Volume 9, Issue 2, March 2007, Pages 152–159
Journal: Metabolic Engineering - Volume 9, Issue 2, March 2007, Pages 152–159
نویسندگان
Isabelle Meynial Salles, Nynne Forchhammer, Christian Croux, Laurence Girbal, Philippe Soucaille,