کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
322715 540220 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glutamate release in the ventromedial hypothalamus of the female rat during copulation: Modulation by estradiol
ترجمه فارسی عنوان
انتشار گلوتامات در هیپوتالاموس ونتومدیال موش ماده در طی زایمان: مدولاسیون توسط استرادیول
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
چکیده انگلیسی


• VMH glutamate inhibits female rat sexual behavior.
• VMH glutamate release was high in hormone-unprimed rats but low in primed rats.
• Ovarian hormones inhibit VMH glutamate release.

Binding of glutamate or its ionotropic receptor agonists in the ventromedial hypothalamus (VMH) of female rats inhibits both appetitive and consummatory aspects of sexual behavior. Because vaginocervical stimulation activates glutamate neurons in the VMH, and administration of estradiol benzoate (EB) and progesterone (P) delays this effect, the present study examined the effects of hormonal priming on glutamate release within the VMH of female rats paired with sexually vigorous males. Ovariectomized, sexually experienced rats were implanted with guide cannula aimed at the ventrolateral VMH, through which microdialysis probes were inserted prior to testing. Females were assigned randomly to one of three hormone treatment conditions: EB + P, EB alone, or the oil vehicle. Testing was conducted over 5 h, including a 120-min period of habituation to the testing chamber, a 60-min period of baseline sample collection, and a 120-min period during which a sexually vigorous male was introduced into the testing chamber. Dialysates were collected every 20 min during the test and were analyzed for glutamate using HPLC. Females primed with oil had large and significant increases in glutamate release from baseline once the male was introduced to the chamber. Treatment with EB alone decreased glutamate release in response to male cues. Although treatment with EB + P did not differ significantly from EB alone, the degree of reduced glutamate release was less than with EB alone. These results indicate that priming with EB reduces glutamate transmission in the VMH in response to male cues. Taken together with our previous findings, estradiol blunts the activation of glutamate neurons in the VMH thus allowing female rats to copulate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hormones and Behavior - Volume 65, Issue 2, February 2014, Pages 119–126
نویسندگان
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