کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
323487 540691 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Central angiotensin II stimulates cutaneous water intake behavior via an angiotensin II type-1 receptor pathway in the Japanese tree frog Hyla japonica
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Central angiotensin II stimulates cutaneous water intake behavior via an angiotensin II type-1 receptor pathway in the Japanese tree frog Hyla japonica
چکیده انگلیسی

Angiotensin II (Ang II) stimulates oral water intake by causing thirst in all terrestrial vertebrates except anurans. Anuran amphibians do not drink orally but absorb water osmotically through ventral skin. In this study, we examined the role of Ang II on the regulation of water-absorption behavior in the Japanese tree frog (Hyla japonica). In fully hydrated frogs, intracerebroventricular (ICV) and intralymphatic sac (ILS) injection of Ang II significantly extended the residence time of water in a dose-dependent manner. Ang II-dependent water uptake was inhibited by ICV pretreatment with an angiotensin II type-1 (AT1) receptor antagonist but not a type-2 (AT2) receptor antagonist. These results suggest that Ang II stimulates water-absorption behavior in the tree frog via an AT1-like but not AT2-like receptor. We then cloned and characterized cDNA of the tree frog AT1 receptor from the brain. The tree frog AT1 receptor cDNA encodes a 361 amino acid residue protein, which is 87% identical to the toad (Bufo marinus) AT1 receptor and exhibits the functional characteristics of an Ang II receptor. AT1 receptor mRNAs were found to be present in a number of tissues including brain (especially in the diencephalon), lung, large intestine, kidney and ventral pelvic skin. When tree frogs were exposed to dehydrating conditions, AT1 receptor mRNA significantly increased in the diencephalon and the rhombencephalon. These data suggest that central Ang II may control water intake behavior via an AT1 receptor on the diencephalon and rhombencephalon in anuran amphibians and may have implications for water consumption in vertebrates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hormones and Behavior - Volume 58, Issue 3, August 2010, Pages 457–464
نویسندگان
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