کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
330644 1433672 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Elevation of cytoskeletal protein breakdown in aged Wistar rat brain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Elevation of cytoskeletal protein breakdown in aged Wistar rat brain
چکیده انگلیسی

Previous studies indicated there is an overall increase of proteolysis in aging rat brains. We monitored the potential degradation of cytoskeletal proteins in neuronal tissue taken from cerebral cortex and cerebellum of young (3 month) and aging (17, 21 and 23.5 month) Wistar rats. We found significant age-dependent proteolysis of cytoskeletal proteins (αII-spectrin and microtubule-associated protein MAP-2A/B) in the cerebral cortex and the cerebellum. The pattern of αII-spectrin breakdown shows a marked increase in 150- and 145-kDa fragments (SBDP150 and SBDP145, respectively), but we did not detect the caspase-3-mediated 120-kDa fragment (SBDP120) in aged rat brains, suggesting the involvement of the calpain proteases. The pattern of MAP-2A/B breakdown in aged rat brains mirrors that produced by in vitro calpain digestion of 3-month control rat brain MAP-2A/B. In aged rat brains, there is no significant increase in pro-caspase-3 processing; rather, there is a moderate reduction in pro-caspase-3 protein and caspase-3 hydrolytic activity in the cortex. These results point to selective susceptibility of cytoskeletal proteins to calpain-mediated degradation, but not caspase-3 in aging rat brains.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Aging - Volume 27, Issue 4, April 2006, Pages 624–632
نویسندگان
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