کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3340515 1214058 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interleukin-17A expression in human synovial mast cells in rheumatoid arthritis and osteoarthritis
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی ایمونولوژی، آلرژی و روماتولوژی
پیش نمایش صفحه اول مقاله
Interleukin-17A expression in human synovial mast cells in rheumatoid arthritis and osteoarthritis
چکیده انگلیسی

BackgroundInterleukin (IL)-17A plays a pivotal role in the pathogenesis of rheumatoid arthritis (RA). The expression of IL-17A in synovial mast cells (MCs) in RA and osteoarthritis (OA) has been reported, but the frequencies of IL-17A expression in synovial MCs have varied. The aim of this study was to investigate whether IL-17A expression is upregulated in human synovial MCs in RA and to elucidate the mechanism of IL-17A expression in synovial MCs.MethodsSynovial tissues were obtained from patients with RA or OA undergoing joint replacement surgery, and synovial MCs were enzymatically dispersed. Synovium-derived cultured MCs were generated by culturing synovial cells with stem cell factor. IL-17A expression was investigated using immunofluorescence in synovial tissues. IL-17A mRNA expression and its production from MCs were examined using RT-PCR and ELISA, respectively.ResultsThe number of IL-17A-positive (+) synovial MCs and the percentage of IL-17A+ MCs among all the IL-17A+ cells from RA patients were not significantly increased compared with those from OA subjects. The synovium-derived cultured MCs spontaneously released small amounts of IL-17A. Neither IgE- nor IgG-dependent stimulation increased IL-17A production from the MCs. IL-33, tumor necrosis factor-α, C5a, lipopolysaccharide or IL-23 plus IL-1β did not affect IL-17A production in MCs.ConclusionsThe synovial MCs are not a main source of IL-17A in RA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Allergology International - Volume 65, Supplement, September 2016, Pages S11–S16
نویسندگان
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