کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
33439 44978 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity
چکیده انگلیسی

3-Hydroxypropionaldehyde (3HPA) is a promising versatile substance derived from the renewable feedstock glycerol. It is a product of glycerol metabolism in Lactobacillus reuteri. Because of toxic effects, the biotechnological production is poor. In this work the biocatalyst lifetime and product formation could be drastically increased. In the established two-step process already applied, cells are grown in the first step under anaerobic conditions, and in the second step the immobilised or suspended biocatalyst is used for 3HPA-production under strict anaerobic conditions. In the first step it was possible to reach a biomass concentration of 5.5 g CDW/L (OD600 ≈ 23.4). In the second step, normally, 3HPA accumulates to a toxic concentration and the reaction stops in less than 60 min because of the interaction of 3HPA with cell components. To prevent this, the toxic product is bound to the newly found scavenger carbohydrazide to form the hydrazone. For the first time it was possible to recycle the immobilised biocatalyst for at least ten cycles (overall life time > 33 hours) in a repeated batch biotransformation with an overall production of 67 g 3HPA. The optimal pH-value was between 6.8 and 7.2 at an optimal temperature of 40–45°C. In a single batch biotransformation with suspended resting cells it was possible to produce 150 g/L 3HPA as carbohydrazone at an overall productivity of 10.7 g L−1 hours−1. In a single fed-batch biotransformation at 45°C 138 g/L glycerol was converted into 108 g/L 3HPA with an overall productivity of 21.6 g L−1 hours−1. This is the highest 3HPA concentration and productivities reported so far for the microbial production of 3HPA from glycerol.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 29, Issue 2, 15 January 2012, Pages 211–217
نویسندگان
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