کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3344123 1591195 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of hepatitis B virus A1762T/G1764A mutant by amplification refractory mutation system
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Detection of hepatitis B virus A1762T/G1764A mutant by amplification refractory mutation system
چکیده انگلیسی

ObjectiveTo study the role of hepatitis B virus with A1762T/G1764A double mutation in liver cirrhosis and hepatocellular carcinoma, and create a sensitive, fast, accurate assay for detection of A1762T/G1764A double mutation.MethodsWe developed an accurate and fast real-time amplification refractory mutation system to detect A1762T/G1764A double mutation. Cloned hepatitis B virus genome was used as a control. Assay sensitivity was determined by serial dilution and mixed template experiments. Specificity was determined by cross experiments with wild and mutant hepatitis B virus. Fifty clinical samples were tested by the real-time amplification refractory mutation system and the results were compared with sequencing.ResultsThe real-time amplification refractory mutation system had a sensitivity of 100 copies of virus with these mutations, and 0.1% weak population virus with double mutation could be found in mixtures. A total of 50 randomly collected clinical samples were detected by real-time amplification refractory mutation system, and the results were consistent with those by DNA sequencing. Hepatitis B virus genotype C was more prevalent in 39 of 50 samples than genotype B (11 samples), and about 75% of genotype C carried a double mutation compared to 45% of genotype B. However, the percentage of A1762T/G1764A double mutation in hepatitis B e antigen-negative (58.3%) samples was almost the same as in hepatitis B e antigen-positive (61%) samples.ConclusionThe real-time amplification refractory mutation system is sensitive and specific for detection of hepatitis B virus double mutation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Brazilian Journal of Infectious Diseases - Volume 18, Issue 3, May–June 2014, Pages 261–265
نویسندگان
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