کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3347139 1215940 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple, sensitive HPLC-PDA method for the quantification of itraconazole and hydroxy itraconazole in human serum: a reference laboratory experience
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
A simple, sensitive HPLC-PDA method for the quantification of itraconazole and hydroxy itraconazole in human serum: a reference laboratory experience
چکیده انگلیسی

A chromatographic method (high-performance liquid chromatography/photodiode array detection) for the simultaneous determination of itraconazole and its mayor metabolite, hydroxy itraconazole, was developed. The validation data demonstrated acceptable values for linearity (dynamic range between 0.25–16 μg/mL for hydroxy itraconazole, 0.125–4 μg/mL for itraconazole), precision (Coefficient of Variation, 2.23–4.28% and 1.61–9.52% respectively), accuracy (Relative Error, −2.89 to −17.26% and −3.11 to 14.44%), selectivity, sensitivity (Lower limit of Quantification, 0.125 and 0.25 μg/mL), and recovery (97.30–105.82%) for both compounds. Its clinical suitability was also investigated in 104 trough human serum samples. The assay resulted to be rapid, sensitive, and simple for simultaneously quantification of these azole compounds in serum samples and should make a positive contribution to optimize therapies on an individual basis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 76, Issue 3, July 2013, Pages 314–320
نویسندگان
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