کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3347959 1215992 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the Abbott RealTime™ CT assay with the BD ProbeTec™ ET assay for the detection of Chlamydia trachomatis in a clinical microbiology laboratory
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Evaluation of the Abbott RealTime™ CT assay with the BD ProbeTec™ ET assay for the detection of Chlamydia trachomatis in a clinical microbiology laboratory
چکیده انگلیسی

The Abbott RealTime™ CT assay (Abbott Molecular, Des Plaines, IL) was evaluated by testing male urine samples (n = 204) and female urine samples (n = 207) with matched endocervical swabs (n = 207) collected from patients attending the Genito-Urinary Infectious Disease Clinic, St. James's Hospital, Dublin, Ireland. Results were compared with the BD ProbeTec™ ET assay (Becton Dickinson, Sparks, MD). Both assays were performed within 3 days of specimen collection. Samples positive with 1 or other assay were subjected to discrepant-based analysis using 2 additional assays, an “in house real-time polymerase chain reaction [PCR]” and a “nested PCR with amplicon sequence detection”. After resolution of discordant results, the Abbott RealTime™ CT assay demonstrated greater clinical sensitivity than the BD ProbeTec™ ET assay for the detection of Chlamydia trachomatis (CT) in all sample types. Both assays demonstrated acceptable analytic sensitivity with detection limits of 22 and 33 cryptic plasmid copies/reaction, respectively. The sensitivity of the Abbott RealTime™ CT assay combined with automated throughput establishes this assay as a quality diagnostic tool.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 64, Issue 1, May 2009, Pages 13–19
نویسندگان
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