کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3348398 1216011 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Consistence of miniexon polymerase chain reaction-restriction fragment length polymorphism and single-copy gene sequence analyses in discriminating Leishmania genotypes
چکیده انگلیسی
Forty-six independent isolates of Leishmania subgenus were examined by restriction fragment length polymorphism (RFLP) analyses of polymerase chain reaction (PCR)-amplified miniexon sequences. The results were consistent with grouping of these isolates into species complexes based on sequence polymorphism of single-copy genes. Miniexon RFLP profiles specific to Leishmania enriettii, Leishmania tarentolae, and Leishmania gerbilli were obtained for the first time. The miniexon PCR-RFLP method is thus further confirmed as a simple, sensitive, and specific method useful for discriminating Leishmania isolates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diagnostic Microbiology and Infectious Disease - Volume 57, Issue 3, March 2007, Pages 295-299
نویسندگان
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