کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3351981 1216448 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulation of human bronchial epithelial cells by pneumococcal choline binding protein A
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Modulation of human bronchial epithelial cells by pneumococcal choline binding protein A
چکیده انگلیسی

Choline binding protein A (CbpA) is an important adhesin and a determinant of virulence for Streptococcus pneumoniae. Binding to epithelial cells of host mucosal surfaces by pneumococcal CbpA is essential for pneumococcus to initiate colonization and to trigger subsequent invasive pneumococcal infections. In this study, we examined the immunopathologic mechanisms for the activation of human bronchial epithelial cells by CbpA in pneumococcal infections. Adhesion molecules, cytokines, and chemokines were assessed by flow cytometry, quantitative real-time polymerase chain reaction, and enzyme-linked immunosorbent assay, respectively. Intracellular signaling molecules were investigated by enzyme-linked immunosorbent assay or transcription factor assay. CbpA could upregulate cell surface expression of adhesion molecule intercellular adhesion molecule–1 on human bronchial epithelial cells. CbpA could also induce the release of inflammatory cytokine interleukin-6, and chemokines CCL2, CXCL1, and CXCL8 from human bronchial epithelia cells. CbpA-mediated induction of these mediators was differentially regulated by extracellular signal-regulated kinase, c-Jun N-terminal protein kinase, p38-mitogen–activated protein kinase, and nuclear factor–κB pathways. CbpA was also found to participate in the induction of IL-6, CCL2, CXCL1, and CXCL8 in the airways of mice upon intranasal challenge with S. pneumoniae. Our study therefore suggests that pneumcoccal CbpA plays an immunopathophysiologic role by activating human bronchial epithelial cells in pneumococcal infections.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 72, Issue 1, January 2011, Pages 37–46
نویسندگان
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