کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3358403 | 1688158 | 2016 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Sequences of a co-existing SXT element, a chromosomal integron (CI) and an IncA/C plasmid and their roles in multidrug resistance in a Vibrio cholerae O1 El Tor strain Sequences of a co-existing SXT element, a chromosomal integron (CI) and an IncA/C plasmid and their roles in multidrug resistance in a Vibrio cholerae O1 El Tor strain](/preview/png/3358403.png)
• Identified a hybrid SXT element (ICEVchCHN1307), a new IncA/C plasmid (pVC1307) and a chromosome integron (CI).
• Provided their complete nucleotide sequence and clearly demonstrated their roles in antibiotic resistance.
• Powerful accumulation of drug resistance genes in Vibrio cholerae.
• More attention to the risk of spreading multiple drug resistances mediated by SXT and the IncA/C plasmid is required.
The ongoing seventh cholera pandemic is attributed to Vibrio cholerae O1 El Tor biotype strains. Although antibiotic therapy ameliorates symptoms in patients and reduces pathogen transfer to the environment, multidrug resistance remains a major clinical threat. An O1 El Tor strain isolated from a patient in 1998 was intermediate or resistant to 13 antibiotics and could potentially produce extended-spectrum β-lactamase (ESBL), which is very rare in O1 strains. Using genome sequencing, three relevant genetic elements were identified in this strain: a hybrid SXT element (ICEVchCHN1307); a new IncA/C plasmid (pVC1307); and a chromosomal integron. Twenty antibiotic resistance genes were located on them, including blaTEM-1, blaCTX-M-14 and phenotypically silenced tetRA genes. These data elucidate the role of individual genetic components in antibiotic resistance and the accumulation of drug resistance genes in V. cholerae.
Journal: International Journal of Antimicrobial Agents - Volume 48, Issue 3, September 2016, Pages 305–309