کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3359850 | 1591839 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vivo selection of imipenem-resistant Klebsiella pneumoniae producing extended-spectrum β-lactamase CTX-M-15 and plasmid-encoded DHA-1 cephalosporinase In vivo selection of imipenem-resistant Klebsiella pneumoniae producing extended-spectrum β-lactamase CTX-M-15 and plasmid-encoded DHA-1 cephalosporinase](/preview/png/3359850.png)
Four Klebsiella pneumoniae isolates (KP1–4) were recovered sequentially from an infected patient. Whilst KP1–3 were resistant to all β-lactams except carbapenems, KP4 recovered after 24 days of imipenem-containing treatment showed additional resistance to carbapenems. No carbapenem hydrolysis could be identified in KP4. Molecular characterisation revealed that KP1–4 were indistinguishable by pulsed-field gel electrophoresis, contained a 95-kb self-transferable plasmid harbouring blaCTXM-15 and blaTEM-1 genes and a 65-kb plasmid that was not transferred by conjugation into Escherichia coli, and harboured the plasmid-mediated blaDHA-1 AmpC β-lactamase gene. In addition, KP4 failed to express OmpK36 owing to a point mutation leading to a premature stop of the protein. This study demonstrates development of carbapenem resistance related to loss of OmpK36 expression in a K. pneumoniae isolate harbouring extended-spectrum β-lactamase and plasmid-mediated cephalosporinase genes following prolonged imipenem exposure.
Journal: International Journal of Antimicrobial Agents - Volume 35, Issue 3, March 2010, Pages 265–268