کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3359855 | 1591839 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Antimicrobial and immunomodulatory activities of an ovine proline/arginine-rich cathelicidin Antimicrobial and immunomodulatory activities of an ovine proline/arginine-rich cathelicidin](/preview/png/3359855.png)
In in vitro co-culture experiments, the ovine-derived cathelicidin OaBac5mini showed antimicrobial activity against Escherichia coli cells and modulated production of a cytokine by a mammalian inflammatory cell type (macrophage). Using atomic force microscopy, the morphology of peptide-treated E. coli bacteria showed no cell lysis, indicating an intracellular mode of action of the peptide leading to bacterial cell inhibition. At a concentration of 50 μg/mL OaBac5mini, the peptide suppressed production of the inflammatory cytokine interleukin-12 by murine J774A cells that had been stimulated with Staphylococcus aureus strain Cowan; levels of other cytokines were unaffected. Thus, certain cationic peptides can enter and disrupt invading Gram-negative pathogens and may be able to modulate inflammatory responses induced by Gram-positive bacterial products.
Journal: International Journal of Antimicrobial Agents - Volume 35, Issue 3, March 2010, Pages 288–291