کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3368773 1592354 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels
چکیده انگلیسی


• Human anelloviruses were detected in all HIV suppressed subjects.
• T-cell activation levels were not correlated with levels of plasma anellovirus.
• T-cell activation levels were not correlated with specific anellovirus groups.
• Anelloviruses do not appear to be a dominant driving force of T-cell activation.

BackgroundAbnormally high levels of T-cell activation can persist in HIV-infected subjects despite effective anti-retroviral therapy (ART) and has been associated with negative health outcomes. The nature of the antigenic drivers or other causes of this residual T-cell activation remain uncertain. Anelloviruses are universally acquired soon after birth, resulting in persistent viremia, and considered part of the commensal human virome. Reduced immunocompetence results in increased anellovirus levels.ObjectivesTo test whether increased levels of anelloviruses or other viruses in plasma are associated with higher levels of persistent T-cell activation during ART.Study designTwo amplification methods combined with next generation sequencing were used to detect all viruses and estimate relative anellovirus levels in plasma from 19 adults on effective ART who exhibited a wide range of T-cell activation levels.ResultsNucleic acids from HBV and HCV were detected in one patient each while pegivirus A (GBV-C) was found in three patients. Anellovirus DNA was detected in all patients with some individuals carrying up to eight different genotypes. Specific anellovirus genotypes or higher level of co-infections were not detected in subjects with higher levels of T-cell activation. No association was detected between relative plasma anellovirus DNA levels and the percentage of activated CD4 or CD8 T cells.ConclusionsHuman anelloviruses were detected in all HIV suppressed subjects, exhibited a wide range of viremia levels, and were genetically highly diverse. The level of persistent T-cell activation was not correlated with the level of viremia or genotypes present indicating that anellovirus antigens are unlikely to be a dominant source of antigens driving chronic T-cell activation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 72, November 2015, Pages 106–113
نویسندگان
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