کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3369930 1219059 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and performance of a new recombinant virus phenotypic entry assay to determine HIV-1 coreceptor usage
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Development and performance of a new recombinant virus phenotypic entry assay to determine HIV-1 coreceptor usage
چکیده انگلیسی

BackgroundClinical trials of CCR5 antagonists have relied on the phenotypic determination of HIV-1 coreceptor usage. Few phenotypic assays are available, with few data on their concordance, and none has been designed to determine tropism from cell-associated HIV-1 DNA.ObjectivesTo assess the performance of the new Toulouse Tropism Test (TTT) phenotypic assay to characterize HIV-1 tropism using blood plasma and peripheral blood mononuclear cells (PBMC).Study design434 plasma and 168 PBMC samples were tested with the TTT assay. We determined the correlation between our assay results on plasma samples and those of the commercial Trofile™ assay.ResultsThe TTT assay determined the tropism of 97% of samples after successful amplification of the env gene. It performed well on both cell samples and plasma samples with various HIV-1 loads and subtypes. It detected 0.5% of minor CXCR4-using variants in the virus population. The TTT and the Trofile™ assays were >90% concordant for predicting HIV-1 tropism.ConclusionWe have validated a new recombinant virus phenotypic assay for determining HIV-1 tropism using both plasma and cell samples from patients who are candidates for treatment with CCR5 antagonists.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 47, Issue 2, February 2010, Pages 126–130
نویسندگان
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