کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3371234 1219110 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trichostatin-A enhances adaptive immune responses to DNA vaccination
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Trichostatin-A enhances adaptive immune responses to DNA vaccination
چکیده انگلیسی

BackgroundThe efficacy of DNA vaccines to date has overall been disappointing, especially in large animal models and in humans, which might be partially explained by the chromatinization of the administered foreign DNA. Trichostatin-A (TSA) is a specific inhibitor for histone deacetylase (HDAC) and a gene expression enhancer in in vitro DNA viral infection, including herpes simplex type 1 (HSV-1) and cytomegalovirus (CMV).ObjectivesWe sought to determine if increasing antigen expression of DNA vaccines through inhibition of HDAC-induced chromatin silencing using TSA would enhance DNA vaccine efficacy in vivo.Study designA luciferase assay was used to detect effects of TSA on different promoters in vitro. The effects of TSA on DNA vaccination of mice were determined by neutralization assays for antibody production and interleukin staining for detection of specific T cell responses.ResultsMice receiving TSA in combination with a DNA vaccine exhibited higher antibody responses to the vaccine than mice not given TSA. Co-administration of TSA also enhanced specific CD8 T cells response.ConclusionDrugs such as TSA that reduce initial gene silencing by preventing histone deacetylation can increase immune responses to DNA vaccination.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 36, Issue 4, August 2006, Pages 292–297
نویسندگان
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