Dengue virus regulates the expression of hemostasis-related molecules in human vein endothelial cells

SummaryObjectivesHemorrhage is considered to be the primary sign of Dengue hemorrhagic fever and Dengue shock syndrome. In the present study we focused on the mechanism in the pro- and anti-coagulation after Dengue virus infection.MethodsThe effects of Dengue virus on the expression and secretion of tissue factor, tissue factor inhibitor, thrombomodulin, tissue plasminogen activator and plasminogen activator inhibitor type-1 in human umbilical vein endothelial cells were detected in this study.ResultsIt was shown that Dengue virus could promote the expression of thrombomodulin in cultured human umbilical vein endothelial cells, thus increasing anticoagulation, and enhancing tissue plasminogen activator activity and therefore increased hemorrhage risk.ConclusionsThe changes above may play an important role in the development of Dengue hemorrhagic fever and Dengue shock syndrome.