کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3376757 1219927 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Diagnostic values for the viral load in peripheral blood mononuclear cells of patients with chronic active Epstein–Barr virus disease
ترجمه فارسی عنوان
مقادیر تشخیصی برای بار ویروسی در سلولهای تک سلولی خون محیطی بیماران مبتلا به بیماری ویروس مزمن اپشتاین بار
کلمات کلیدی
بیماری ویروسی اپشتاین بار بارور مزمن؛ CAEBV؛ بار ویروسی؛ تشخیص؛ سلول های تک هسته ای خون محیطی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی

Chronic active Epstein–Barr virus disease (CAEBV) is a distinct EBV-associated lymphoproliferative disease with a poor prognosis. Although the viral load in blood samples has been widely used for diagnosing CAEBV, well-defined viral load thresholds to guide clinicians are currently lacking. The aim of the present study was to determine standardized diagnostic values for EBV load in blood samples of CAEBV patients using the World Health Organization international standard for reporting. Levels of EBV DNA in 103 peripheral blood mononuclear cells (PBMCs) and 95 plasma/serum samples from 107 cases with CAEBV were quantified and expressed in international units. Receiver operating characteristic curves were analyzed to assess the most appropriate cut-off values for levels of EBV DNA to distinguish CAEBV from EBV-associated infectious mononucleosis (IM) and controls with past EBV infection. Levels of EBV DNA in PBMCs were significantly higher in the CAEBV group (median, 104.2 IU/μgDNA) compared to the IM (median, 102.1 IU/μgDNA) and control groups. An inconsistent qualitative result was seen in 13 of 86 CAEBV patients; in these, EBV-DNA was positive in PBMCs, but negative in plasma. Diagnostic cut-off values for viral load in PBMCs from CAEBV patients, as compared to those of healthy controls and IM patients, were 102.0 IU/μgDNA and 103.2 IU/μgDNA, respectively. For diagnostic purposes, the viral load of PBMCs was better than of plasma/serum. A diagnostic cut-off EBV load for CAEBV may be useful for the management of CAEBV patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Infection and Chemotherapy - Volume 22, Issue 4, April 2016, Pages 268–271
نویسندگان
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