کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3378044 1220060 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Coaggregation between Prevotella oris and Porphyromonas gingivalis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Coaggregation between Prevotella oris and Porphyromonas gingivalis
چکیده انگلیسی

Background/PurposeThe coaggregation of bacteria has been defined as one of the most important processes in the oral infection such as periodontitis. Prevotella oris and Porphyromonas gingivalis, which are two of the periodontopathogens, are frequently detected in severe forms of periodontal diseases. However, the interaction between P. oris and P. gingivalis is still unknown. In this study, the coaggregation of P. oris with nine oral bacterial species including P. gingivalis was examined.MethodsAll bacteria used in this study were cultured anaerobically and suspended in coaggregation buffer. Each cell suspension was mixed in a test tube and subjected to shaking at room temperature for 1 hour. Subsequently, the coaggregation values were scored. Furthermore, the effects of various chemical reagents, and heat, proteinase K, and serum treatment were examined.ResultsIn this study, P. oris coaggregated only with P. gingivalis. A heat-stable, nonproteinous component of P. oris and a heat-labile, proteinous component of P. gingivalis play important roles in this coaggregation. In addition, this coaggregation was inhibited by l-arginine, l-lysine, and Nα-p-tosyl-l-lysine. Therefore, it was considered that a cell surface protein on P. gingivalis, such as gingipain, may be involved in the coaggregation. Furthermore, the coaggregation was not inhibited by serum treatment.ConclusionThis is the first report to describe the coaggregation of P. oris and P. gingivalis. Our study proposes the possibility that P. oris may promote the colonization of P. gingivalis in an early stage of biofilm formation. Furthermore, this coaggregation may contribute to the initiation and progression of periodontitis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiology, Immunology and Infection - Volume 47, Issue 3, June 2014, Pages 182–186
نویسندگان
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