کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3395 | 168 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Production of (R)-epichlorohydrin from 1,3-dichloro-2-propanol by two-step biocatalysis using haloalcohol dehalogenase and epoxide hydrolase in two-phase system Production of (R)-epichlorohydrin from 1,3-dichloro-2-propanol by two-step biocatalysis using haloalcohol dehalogenase and epoxide hydrolase in two-phase system](/preview/png/3395.png)
Recombinant Escherichia coli cells harbouring haloalcohol dehalogenase and epoxide hydrolase were successfully immobilized by adsorption onto perlite and used to prepare (R)-epichlorohydrin from 1,3-dichloro-2-propanol by two-step biocatalysis in a specially designed reactor. Two-phase solution was used as the reaction system in order to improve the yield of epichlorohydrin. In the two-phase system containing 40% (v/v) cyclohexane, the yield of racemic epichlorohydrin formed in the first step was 73%, and the yield of (R)-epichlorohydrin with enantiomeric excess (ee) ≥99% increased from 19.2% to 25.1% in the second step. Ultimately, the yield of (R)-epichlorohydrin reached 26.4% by optimization of the flow rate of air and amount of immobilized cells. To our knowledge, this was the first report on production of (R)-epichlorohydrin from 1,3-dichloro-2-propanol by two-step biocatalysis using haloalcohol dehalogenase and epoxide hydrolase.
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► (R)-Epichlorohydrin from 1,3-dichloro-2-propanol was prepared by two-step biocatalysis.
► Haloalcohol dehalogenase and epoxide hydrolase were successfully immobilized on perlites.
► A special reactor was designed for eliminating the racemisation of epichlorohydrin.
► Two-phase solution was used to reduce the spontaneous chemical hydrolysis and reverse reaction of epichlorohydrin.
Journal: Biochemical Engineering Journal - Volume 74, 15 May 2013, Pages 1–7