کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
34095 45002 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of gene expression by RNase P
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Inhibition of gene expression by RNase P
چکیده انگلیسی

The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 27, Issue 3, 31 July 2010, Pages 212–221
نویسندگان
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