کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
34181 45008 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel biocatalysts for glycerol conversion into 2,3-butanediol
ترجمه فارسی عنوان
زیست کاتالیزورهای جدید برای تبدیل گلیسرول به 2،3-بوتانیدول
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Conversion of pure and raw glycerol to 2,3-butanediol with safe strains is studied.
• Screening of 850 isolated strains from biodiesel factories yielded two candidates.
• Batch bioprocesses optimization with the Raoultella candidates was undertaken.
• Taguchi strategy for experimental design and result comparison was employed.
• R. terrigena and R. planticolla strains proved to be efficient.

Bioconversion of biodiesel-derived waste glycerol into high-value products is proposed as a solution to improve economic viability of biorefineries. Several microorganisms are able to metabolize glycerol into 2,3-butanediol (2,3-BD), a promising bulk chemical with wide applications: solvent, fuel additive, and feedstock for synthetic rubber production, among them. In the present work, a wide screening of microorganisms present both into the waste water treatment system in a biodiesel industry and in culture collections was carried out in order to evaluate their potential as new 2,3-BD producer biocatalysts. Two microorganisms for 2,3-BD production from glycerol as sole carbon source have been selected, namely Raoultella planticola CECT 843 and Raoultella terrigena CECT 4519. Raoultella strains belong to the non‐pathogenic bacteria class (biosafety level 1). This genus has not been previously described as biocatalyst for the studied process. The influence of operational temperature, organic acid addition, and yeast extract concentration on 2,3-BD yield and productivity have been studied through Taguchi design methodology as well as initial glycerol concentration. Based on these results, the feasibility of the process employing pure glycerol and different samples of raw glycerol has been demonstrated.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 51, Issue 6, June 2016, Pages 740–748
نویسندگان
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