کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3421140 | 1594009 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
A real-time PCR method targeting the small subunit of the rRNA gene was developed for the detection of Strongyloides stercoralis DNA in faecal samples, including an internal control to detect inhibition of the amplification process. The assay was performed on a range of well-defined control samples (n = 145), known positive faecal samples (n = 38) and faecal samples from a region in northern Ghana where S. stercoralis infections are highly endemic (n = 212), and achieved 100% specificity and high sensitivity. The use of this assay could facilitate monitoring the prevalence and intensity of S. stercoralis infections during helminth intervention programs. Moreover, the use of this assay in diagnostic laboratories could make the introduction of molecular diagnostics feasible in the routine diagnosis of S. stercoralis infections, with a two-fold increase in the detection rate as compared with the commonly used Baermann sedimentation method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 103, Issue 4, April 2009, Pages 342-346
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 103, Issue 4, April 2009, Pages 342-346
نویسندگان
Jaco J. Verweij, Marco Canales, Katja Polman, Juventus Ziem, Eric A.T. Brienen, Anton M. Polderman, Lisette van Lieshout,