کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3421477 | 1594032 | 2007 | 8 صفحه PDF | دانلود رایگان |
SummaryWe describe here a method, potentially suitable for field applications, for semi-quantitative detection of Mycobacterium leprae antigens in skin scrapings, which are taken normally for smear microscopy. Thirty acid-fast bacilli-negative paucibacillary (PB) leprosy patients comprised the main study group; eight acid-fast bacilli-positive multibacillary (MB) patients and five healthy laboratory workers served as controls. Samples in saline were spotted on nitrocellulose paper and probed with mycobacterium-specific polyclonal or M. leprae-specific mAbs against 12, 35 and 65 kDa protein antigens, using a dot-ELISA format. Spot densities were read through a densitometer and also graded visually. The polyclonal antibody produced the best sensitivity, resulting in densitometric detection of mycobacterial antigen in 100% MB, 76% multiple-lesion PB and 62% single-lesion PB patients. None of the healthy volunteers showed antigen positivity. A correlation was noted between the densitometric and visual estimates of the antigen. Determination of antigen in the lesion and an apparently uninvolved area of skin in a subset of PB patients provided clues to the state of the underlying infection. Serological positivity of PB patients for M. leprae-specific antibodies against the 35 kDa and phenolic glycolipid-I antigens was too low (<20%) for any diagnostic significance.
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 101, Issue 7, July 2007, Pages 699–706