کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
35168 | 45079 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and localization of the endothelin receptors in human end-stage heart failure
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Characterization and localization of the endothelin receptors in human end-stage heart failure Characterization and localization of the endothelin receptors in human end-stage heart failure](/preview/png/35168.png)
چکیده انگلیسی
Endothelin (ET) is a potent vasoconstrictor peptide implicated in numerous human diseases, including ischemic cardiomyopathy (ICM). ET binds to receptors ETA and ETB. Specific ET receptors were characterized in left atria of patients with end-stage heart failure due to ischemic cardiomyopathy (ICM) (n = 9) and healthy controls (n = 9). Saturation assays revealed a Kd and Bmax of 28 ± 8 pM and 87 ± 22 fmol mgâ1 of protein, respectively, from healthy atria and 50 ± 9 pM and 162 ± 19 fmol mgâ1 of protein, respectively, from diseased atria (p < 0.05). For competition studies, we obtained a percentage of ETA receptors using BQ123, 55% ± 5 and 66% ± 4 in healthy and diseased atria, respectively (p < 0.05). The percentage of ETB using BQ3020 was 55% ± 14 in healthy and 59% ± 10 in diseased atria. Microautoradiography studies showed a greater number of ET receptors, predominately ETA, existed in the myocardial layer of diseased atria compared with healthy atria. The percentage of occupied area was greater in the endocardium than the epicardium in diseased atria. These results showed an increase in the ETA/ETB receptor system in the failing human heart compared with the non-failing human heart.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 44, Issue 6, June 2009, Pages 661-667
Journal: Process Biochemistry - Volume 44, Issue 6, June 2009, Pages 661-667
نویسندگان
M. Rosa Bernal-López, Ainhoa RÃpodas, Paloma Aragoncillo, Amparo Carbonell, Juan José Rufilanchas, Raquel Fernández-Durango, Francisco J. Tinahones, Ricardo Gómez-Huelgas, Arturo Fernández-Cruz,