Enzymatic tannase treatment of green tea increases in vitro inhibitory activity against N-nitrosation of dimethylamine

In vitro experiments were performed to test inhibition of nitrite-mediated N-nitrosation by individual catechins, green tea, and tannase-treated green tea extracts. The extent of inhibition was measured via nitrosamine formation. Green tea with or without tannase treatment was examined to study nitrosation inhibition in order to evaluate the inhibitory activities with the structural changes of catechins present in the extracts. The results showed that the tannase-treated green tea had a greater ability to inhibit the nitrosation than green tea and ascorbic acid did. The tannase-treated green tea strongly inhibited the formation of N-nitrosodimethylamine (NDMA). Among four major catechins tested, epigallocatechin blocked the N-nitrosation efficiently, and epigallocatechin gallate was more unstable than epigallocatechin at pH 2.0 or 8.0. These results suggest that the consumption of tannase-treated green tea can reduce NDMA formation.