کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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35738 | 45104 | 2008 | 8 صفحه PDF | دانلود رایگان |
A fungal immunomodulatory protein (FIP-gts) was purified from Ganoderma tsugae. Recombinant FIP-gts was expressed as glutathione S-transferase fusion protein in E. coli. In this study, recombinant FIP-gts promoted cell cycle progression from G0/G1 to S phase and signaling pathways were investigated in human peripheral mononuclear cells (HPBMCs) by PI staining and flow cytometry. FIP-gts significantly induced cytokine interferon-gamma (IFN-γ) expression. Pre-treatment of cells with phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor LY294002 abolished FIP-gts, induced increase in S phase, and inhibited IFN-γ secretion. Akt, a downstream effector of PI 3-kinase was phosphorylated and activated by FIP-gts. Results show that FIP-gts is a potent activator in PBMC and its effects are mediated via cytokine regulation of PI 3-kinase.
Journal: Process Biochemistry - Volume 43, Issue 4, April 2008, Pages 423–430