کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
35792 | 45106 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Manipulation of B. megaterium growth for efficient 2-KLG production by K. vulgare Manipulation of B. megaterium growth for efficient 2-KLG production by K. vulgare](/preview/png/35792.png)
In the two-step Vitamin C fermentative production, its precursor 2-keto-l-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The rates of K. vulgare cell growth and 2-KLG production were closely related with B. megaterium concentration in the co-culture system. To enhance the 2-KLG production efficiency, a strategy of manipulating B. megaterium growth in the co-culture system and properly releasing its intracellular components was introduced. Lysozyme was used specifically to damage B. megaterium cell wall structure and subsequently inhibit its cell growth. When 10,000 U mL−1 lysozyme was fed to the co-culture system at 12 h, the growth rate of K. vulgare, sorbose consumption rate, and 2-KLG productivity could increase 27.4%, 37.1%, and 28.2%, respectively.
Journal: Process Biochemistry - Volume 45, Issue 4, April 2010, Pages 602–606