Ram horn hydrolysate as enhancer of xanthan production in batch culture of Xanthomonas campestris EBK-4 isolate

Ram horns are waste materials from the meat industry. The objective of this study was to investigate the effects of various concentrations of ram horn (RHH) hydrolysate as a supplement on xanthan production from a local isolate of Xanthomonas campestris EBK-4 in batch culture. Firstly, ram horn hydrolysate was reproduced. The production of xanthan was influenced by the RHH. RHH supplementation promoted X. campestris growth, accelerated substrate metabolism, and increased xanthan production. A concentration of 3% v/v RHH resulted in the highest xanthan concentration (25.6 g/L) in 48 h. This value was 49% higher than that of control medium (17.1 g/L) in the absence of RHH in 60 h. The pyruvate content increased with increasing RHH concentrations. The application of RHH resulted in enhancement of xanthan production.