کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35939 45115 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Purification of xylanase from alkaliphilic Bacillus sp. K-8 by using corn husk column
چکیده انگلیسی

The objective of this work was to apply low cost materials, agricultural residues, to the purification of xylanase. The results showed that crude extracellular, cellulase-free xylanase of an alkaliphilic Bacillus sp. strain K-8 could be purified in a single step by affinity adsorption–desorption on a corn husk column using a high flow rate, under the conditions 25 mM acetate buffer, pH 4.0, 4 °C, which prevented the hydrolysis of xylan by xylanase. After adsorption, the xylanase was eluted from the enzyme–corn husk complex with 500 mM Urea. The enzyme was purified 5.3-fold to homogeneity from culture supernatant. The molecular weight of the purified enzyme was 24 kDa as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The specific activity and recovery yield after purification were 25.4 U/mg protein and 42.3%, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 41, Issue 12, December 2006, Pages 2441–2445
نویسندگان
, , ,