کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
35984 45117 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aureobasidium pullulans batch cultivations based on a factorial design for improving the production and molecular weight of exopolysaccharides
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Aureobasidium pullulans batch cultivations based on a factorial design for improving the production and molecular weight of exopolysaccharides
چکیده انگلیسی

Six factors: strain, carbon source, nitrogen source, nitrogen concentration, aeration, and initial pH, were investigated for their effects on exopolysaccharides (EPS) production in Erlenmeyer flasks by Aureobasidium pullulans using 2-level fractional factorial design. The concentration and molecular weight (MW) of EPS were optimized simultaneously. The effects of main factors together with possible two-factor interactions were detected, and the levels of the six factors were optimized using empirical models. Analysis of factor effects revealed that strain had the strongest influence on EPS concentration, and nitrogen source on MW of EPS. However, nitrogen concentration did not show significant influence on both parameters. The influences of factors on the production variability were also monitored for quality control purposes. At the optimum levels of control factors investigated, as high as 22.6 g/L EPS with a weight average MW of higher than 2 × 106 was produced. The results of this work indicate that the production kinetics varies for different microorganism–medium–environment systems.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 42, Issue 5, May 2007, Pages 820–827
نویسندگان
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