کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
36243 | 45125 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools](/preview/png/36243.png)
چکیده انگلیسی
Statistical experimental designs were applied for the optimization of cell-bound phytase production by Pichia anomala in a cost-effective cane molasses medium. Using Plackett–Burman design, cane molasses, urea and inoculum density were identified as significant factors and these factors were subsequently optimized using a central composite design. The optimum variables that supported maximum enzyme titre were 8% cane molasses, 0.4% urea and 2% inoculum density. The validity of the optimized variables was verified in shake-flasks as well as in a laboratory fermenter. An overall 5- and 1.6-fold enhancement in phytase titres (324–1689 U/g DYB) and biomass production (6.4–10 g/l) were attained due to optimization, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3095–3102
Journal: Process Biochemistry - Volume 40, Issue 9, September 2005, Pages 3095–3102
نویسندگان
Parvinder Kaur, T. Satyanarayana,