کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
36687 45142 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production
چکیده انگلیسی

The high cell density fermentation of recombinant Pichia pastoris for human serum albumin (HSA) production is a high oxygen demand process. The oxygen demand is usually met by increased agitation rate and use of oxygen-enriched air. However, fermentation using a microbubble dispersion (MBD) can supply adequate oxygen to the microorganisms at relatively low agitation rates because of improved oxygen mass-transfer of the microbubbles used for the sparging. Conventional air-sparged fermentations were conducted for the production of HSA using P. pastoris at agitation rates of 350, 500, 750 rpm. These experiments were compared to MBD-sparged fermentation at 350 and 500 rpm agitation rates. After 97 h of air-sparged fermentation, cell masses were 27.9, 48.9, and 138.2 g/l at agitation rates of 350, 500, and 750 rpm, respectively. For MBD-sparged fermentation, cell mass concentrations after 97 h were 129.9 and 137.7 g/l at 350 and 500 rpm, respectively. Protein concentrations were 36.3 and 67.8 mg/l for air-sparged at 350 and 500 rpm, while 267.3 and 301.2 mg/l for MBD-sparged experiments at 350 and 500 rpm, respectively. The volumetric oxygen mass-transfer coefficient, kLa for the MBD system at 500 rpm was 268.6 h−1 compared to 76.1 h−1 for the conventional sparger at 500 rpm. There was significant improvement in oxygen transfer to the microorganism relative to the conventional sparger.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Process Biochemistry - Volume 40, Issue 6, May 2005, Pages 2073–2078
نویسندگان
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