کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3840739 | 1247933 | 2011 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Redistribution of labile plasma zinc during mild surgical stress in the rat
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
پزشکی و دندانپزشکی (عمومی)
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چکیده انگلیسی
Zinc is an essential trace element and cofactor for many cellular processes. Uptake of ionized divalent zinc (Zn2+) in peripheral tissues depends on its total content in the circulation and on mechanisms facilitating delivery to tissues in its labile form. Understanding mechanisms of Zn2+ delivery has been hindered by the absence of techniques to detect labile Zn2+ in the circulation. In this study, we report the use of the fluorescent zinc-binding dye (ZnAF-2) to detect changes in labile Zn2+ in the circulating plasma of the rat under standardized conditions, including exogenous infusions to increase plasma Zn2+ and an infusion of the chelator, citrate, to decrease labile Zn2+ in the plasma without altering total Zn2+ content. In a model of mild surgical stress (unilateral femoral arterial ligation), plasma levels of total and labile Zn2+ decreased significantly 24 h after the operation. Ultrafiltration of plasma into high- and low-molecular weight macromolecule fractionations indicated that binding capacity of zinc in the high-molecular weight fraction is impaired for the entire 24-h interval after induction of mild surgical stress. Affinity of the filtrate fraction was rapidly and reversibly responsive to anesthesia alone, decreasing significantly at 4Â h and recovering at 24 h; in animals subjected to moderate surgical stress, this responsiveness was lost. These findings are the first reported measurements of labile Zn2+ in the circulation in any form of mild systemic stress. Zinc undergoes substantial redistribution in the plasma as a response to surgical stress, leading to increased availability in lower molecular weight fractions and in its labile form.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 157, Issue 3, March 2011, Pages 139-149
Journal: Translational Research - Volume 157, Issue 3, March 2011, Pages 139-149
نویسندگان
Edward Kelly, Jeffrey Mathew, Jonathan E. Kohler, Amy L. Blass, David I. Soybel,