کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3919380 1599783 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Caspase activity and oxidative stress of granulosa cells are associated with the viability and developmental potential of vitrified immature oocytes
ترجمه فارسی عنوان
فعالیت کاسپاز و استرس اکسیداتیو سلولهای گرانولوزا با پتانسیل زنده گیی و رشد انباشته تخمک های نابالغ
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
چکیده انگلیسی

ObjectiveThe aim of this study is to determine whether caspase activity and oxidative stress of granulosa cells are associated with the viability and developmental potential of vitrified immature oocytes.Study designOocytes from mice were exposed to genistein or/and Z-VAD-FMK with or without vitrification. Ultrastructural alterations of granulosa cells in vitrified immature oocytes were observed. Moreover, the level of superoxide dismutase (SOD) in granulosa cells, incidence of apoptotic follicles, the viability of vitrified-warmed oocytes and their subsequent developmental competence were measured.ResultsUltrastructural alterations of granulosa cells vitrified in the presence of genistein or Z-VAD-FMK were slighter than that of granulosa cells vitrified in the absence of genistein or Z-VAD-FMK. The incidence of apoptotic follicles vitrified in the presence of genistein or Z-VAD-FMK was significantly lower than that of immature oocytes vitrified in the absence of genistein or Z-VAD-FMK, whereas, the level of SOD in granulosa cells, the viability and developmental competence of immature oocytes vitrified in the presence of genistein or Z-VAD-FMK were significantly higher than that of immature oocytes vitrified in the absence of genistein or Z-VAD-FMK.ConclusionBoth antioxidant (genistein) and caspase inhibition (Z-VAD-FMK) improve the viability and developmental competence of vitrified immature oocytes. Genistein is superior to Z-VAD-FMK in improving the efficacy of immature oocyte vitrification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Obstetrics & Gynecology and Reproductive Biology - Volume 198, March 2016, Pages 22–26
نویسندگان
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