کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3934930 1253394 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of automated imaging and analysis technology for the detection of aneuploidy in human sperm
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
Use of automated imaging and analysis technology for the detection of aneuploidy in human sperm
چکیده انگلیسی

ObjectiveTo determine the precision and accuracy of an automated cell counting system when applied to counting aneuploidies in sperm samples.DesignProspective pilot study.SettingAndrology clinic and research laboratory in a university teaching hospital.Patient(s)Ten anonymous sperm donors of known fertility and two patients seeking infertility treatment.Intervention(s)Semen samples were processed for detection of aneuploidies for chromosomes 13, 18, 21, X, and Y with use of fluorescent in situ hybridization. The detection of chromosome aneuploidy was performed both by manual counting and by the use of an automated cell counting system with manual review of aneuploid sperm.Main Outcome Measure(s)Semen samples were judged for the percent aneuploidy for chromosomes 13, 18, 21, X, and Y when counted manually or with the use of the automated cell counting system and review by a technician.Result(s)The sperm aneuploidy rates determined by the automated cell counting system and careful review were comparable with those obtained by manual counting by a trained technician.Conclusion(s)These preliminary data demonstrate that automated cell counting devices may be useful in increasing productivity in aneuploidy detection in sperm and may become an alternative to the labor-intensive manual counting by technicians.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fertility and Sterility - Volume 90, Issue 2, August 2008, Pages 434–437
نویسندگان
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