کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4011281 1602619 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Aqueous humor oxidative stress proteomic levels in primary open angle glaucoma
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Aqueous humor oxidative stress proteomic levels in primary open angle glaucoma
چکیده انگلیسی

The purpose of this work was to investigate the expression of glutamine synthase (GS), nitric oxide synthase (NOS) superoxide dismutase (SOD) and glutathione transferase (GST) in the aqueous humor of patients with primary open angle glaucoma and controls. Aqueous humor proteome was analyzed by antibody microarray. The expression of tested proteins was detected by protein Cy3/Cy5 labeling, column purification and hybridization on antibody-spotted glass microarray. Fluorescent signals were detected by fluorescence laser scanning. Aqueous humor levels of SOD as well as of GST were significantly lower (2.0- and 2.2-fold, p < 0.01) among patients than controls; both NOS and GS expression were significantly higher (2.2- and 2.6 fold, p < 0.01) among patients than controls. Our data showed substantial differences of GS, NOS2, SOD and GST aqueous humor levels between glaucomatous patients and controls as measured by antibody microarray technology. The overproduction of NO through inducible NOS can form toxic products and change the metabolic conditions of the TM. The GS over-expression might be related to neuronal injury or to the potential role of glutamate as a modulator in the ciliary body signaling. The reduced expression of the antioxidant enzymes SOD and GST could aggravate the unbalance between both oxygen- and nitrogen-derived free radicals production and detoxification. Based on our results, GS, NOS2, SOD and GST as measured by antibody microarray technology may be useful oxidative markers in aqueous humor of glaucomatous patients.


► Aqueous humor proteome showed differences between POAG patients and controls.
► Antibody microarray arises as an innovative approach to aqueous humor analysis.
► GS as well NOS2 levels were higher among POAG than controls.
► SOD as well GST levels were lower among POAG than controls.
► These enzymes could be useful biomarkers for glaucoma detection and treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 103, October 2012, Pages 55–62
نویسندگان
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