کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4012694 1261205 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in the localization of collagens IV and VIII in corneas obtained from patients with posterior polymorphous corneal dystrophy
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی و میکروب شناسی (عمومی)
پیش نمایش صفحه اول مقاله
Changes in the localization of collagens IV and VIII in corneas obtained from patients with posterior polymorphous corneal dystrophy
چکیده انگلیسی

Posterior polymorphous corneal dystrophy (PPCD) is a hereditary bilateral disorder affecting primarily the endothelium and Descemet's membrane (DM). The aim of this study was to determine the changes in the presence and localization of the α1–α6 collagen IV chains and α1, α2 collagen VIII chains in Czech patients with PPCD. Twelve corneal buttons from ten PPCD patients who underwent corneal grafting, as well as eight unaffected corneas, were used. Enzymatic indirect immunohistochemistry was performed on cryosections using antibodies against the α1–α6 collagen IV chains and α1, α2 collagen VIII chains. The intensity of the signal was examined separately in the basal membrane of the epithelium (BME), stroma and DM. More than 50% of PPCD specimens exhibited positivity for α1 and α2 collagen IV chains in the BME and in the posterior stroma, while no staining was detected in these areas in control specimens. The signal for the α1 and α2 collagen IV chains was more intense in DM of PPCD corneas compared to controls and it was shifted from the stromal side (in control tissue) to the endothelial side of DM (in the patients). A less intensive signal in PPCD corneas for the α3 and α5 chains in DM and an accumulation of α3–α5 in the posterior stroma in diseased corneas were the only differences in staining for the α3–α6 collagen IV chains. The α1 collagen VIII chain was detected on both the endothelial and the stromal sides of DM in 90% of patients with PPCD, compared with the prevailing localization on the stromal side of DM in control corneas. A change in the localization of the α2 collagen VIII chain in DM from vertically striated features in control specimens to double line positivity in the DM of PPCD corneas and positive staining in the posterior collagenous layer of four patients were also detected. In three PPCD patients a fibrous pannus located under the BME, positive for α1–α3, α5 collagen IV chains and α1 collagen VIII chain, was observed. The increased expression of the α1, α2 collagen IV and α1 collagen VIII chains and the change in their localization in DM may contribute to the increased endothelial proliferative capacity observed in PPCD patients.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Eye Research - Volume 88, Issue 5, May 2009, Pages 945–952
نویسندگان
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