کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4138658 | 1272163 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Histochemical identification of Methicillin-resistant Staphylococcus aureus: contribution to preventing nosocomial infection
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
آسیبشناسی و فناوری پزشکی
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چکیده انگلیسی
Histopathological diagnosis of bacterial infection remains a technical challenge. Application of histochemistry provides a prospect of the improvement of diagnostic quality. Methicillin-resistant Staphylococcus aureus (MRSA), the most common drug-resistant bacterium, is of clinical importance in making appropriate histopathological diagnosis. Recently, community-acquired MRSA has expanded, in addition to conventional hospital-acquired MRSA. Immunohistochemical identification of MRSA requires antibodies against both species-specific antigens and penicillin-binding protein 2â² (PBP2â²), whereas a species-specific gene and mec A encoding PBP2â² can be the target of in situ hybridization (ISH) detection. Specificity verification in histochemistry should be emphasized, since S. aureus commonly expresses protein A in the cell wall, whose immunoglobulin-binding capacity is retrieved by heating pretreatment of routinely prepared sections. The ISH technique for MRSA needs meticulous pretreatment of routine paraffin-embedded sections and signal enhancement sequence. This review focuses on such histopathological approaches, which should have profound potential for contributing to decreasing and preventing nosocomial infection of MRSA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Seminars in Diagnostic Pathology - Volume 24, Issue 4, November 2007, Pages 217-226
Journal: Seminars in Diagnostic Pathology - Volume 24, Issue 4, November 2007, Pages 217-226
نویسندگان
Ryoichi MD, Yutaka MD,