کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4160602 1273848 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Absent STAT-1 expression perturbs adaptation and apoptosis after massive intestinal resection
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پریناتولوژی (پزشکی مادر و جنین)، طب اطفال و بهداشت کودک
پیش نمایش صفحه اول مقاله
Absent STAT-1 expression perturbs adaptation and apoptosis after massive intestinal resection
چکیده انگلیسی

BackgroundWe have previously established the significance of epidermal growth factor receptor (EGFR) activity and the cyclin-dependent kinase inhibitor p21waf1/cip1 (p21) for the adaptive response of the intestine to massive small bowel resection (SBR). In this study, we tested the role of the signal transducer and activator of transcription 1 (STAT-1) as this transcription factor is activated by the EGFR and known to induce p21 expression.MethodsControl (n = 40; C57/Bl6) and STAT-1–null mice (n = 40) underwent 50% proximal SBR or sham operation. After 3 days, the remnant ileum was harvested and the villus and crypt morphology was measured along with changes in rates of enterocyte proliferation and apoptosis.ResultsThe magnitude of resection-induced adaptation was greater in STAT-1–null animals as verified by taller villi and deeper crypts. The expected increase in enterocyte apoptosis did not occur after SBR in the background of STAT-1 deficiency. Western blotting revealed elevated expression of p21 protein in both STAT-1–null and controls after SBR.ConclusionIncreased p21 expression after SBR in the absence of STAT-1 suggests an alternate mechanism for resection-induced regulation of p21. Enhanced adaptation in STAT-1–null animals suggests that this transcription factor serves an inhibitor to the process of adaptation, perhaps via regulation of enterocyte apoptosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pediatric Surgery - Volume 41, Issue 4, April 2006, Pages 713–718
نویسندگان
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