کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4167 212 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Micellar electrokinetic chromatography after precapillary enzyme assay for the determination of phosphatase activity in semiarid soil
چکیده انگلیسی

Inorganic phosphorous (P) is an essential plant nutrient that must be added to most soils to maintain plant growth and sustain crop yields. Phosphatase enzymes play a major role in the processes of mineralization of organic P substrates. In the present study we propose a precise and accurate method to determine the alkaline and acid phosphatase activity in soil using adenosine monophosphate (AMP) as a substrate. The adenosine released by the enzymatic reaction was quantified by micellar electrokinetic chromatography (MEKC) method. The background electrolyte (BGE) consisted in 20 mM phosphate buffer, 10 mM sodium dodecyl sulphate (SDS) and 10% acetonitrile (ACN). The LOD and LOQ for adenosine were 2.27 × 10−4 and 6.4 × 10−4 mg mL−1, respectively. This sensitive method allowed the use of natural compound as enzymatic substrate, which is commonly found in soil. Moreover, we improve the detection method by diminishing the amount of waste and using a harmless substrate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 46, Issue 2, 1 October 2009, Pages 121–125
نویسندگان
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